Table 3.
Variations within genes related to biosynthesis of secondary metabolites
| Gene locus in NRRL 2338 | Length (amino acids) |
Gene (locus) | Variationa | Protein function and notes |
|---|---|---|---|---|
| Polyketide synthases and related biosynthetic proteins | ||||
| SACE_0019 | 95 | (pfa) | Nonsense (Q86*) | Acyl carrier protein |
| SACE_0022 | 752 | pfaB (pfa) | Frameshift (+T 2003/2004) | Modular polyketide synthase (pfa gene cluster) (module 1: KR-ACP) |
| SACE_0023 | 2322 | pfaC (pfa) | Missense (A317T,D1707N) | Modular polyketide synthase (pfa gene cluster) (module 2: KS-TE) |
| SACE_0718 | 237 | eryCVI (ery) | Missense (T64A) | Erythromycin biosynthesis: TDP-desosamine-N-dimethy transferase |
| SACE_0720 | 322 | eryBIV (ery) | Missense (G3E) | Erythromycin biosynthesis: dTDP-4-keto-6-deoxy-L-hexose 4-reductase |
| SACE_2595 | 2368 | Missense (G1215S, D1118N) | Type I polyketide synthase (module: KS-AT-DH-ER-KR-ACP) | |
| SACE_2630 | 4576 | pks2-l (pks2) | Missense (A3004V) | Modular polyketide synthase (module 1: KS-AT(P)-ACP; module 2: KS-AT(A)-DH-KR-ACP; module 3: KS-AT(A)-DH-KR-ACP) |
| SACE_2875 | 4132 | (pks3) | Frameshift (-G 2035) | Modular polyketide synthase (pks3 gene cluster (load: CL-ACP; module 1: KS-AT(P)-DH-ER-KR-ACP; module 2: KS-AT(A)-ACP) |
| SACE_2876 | 252 | gdmF (pks3) | Missense (G17W) | 3-amino-5-hydroxybenzoic acid synthase (AHBA synthase) |
| SACE_2888 | 455 | Missense (V91C) | Aromatic-L-amino-acid decarboxylase | |
| SACE_4140 | 3481 | pkeA2 (pke) | Missense (F1282V) | Modular polyketide synthase (module 1: KS-AT(A)-DH-KR-ACP; module 2: KS-AT(A)-DH-KR-ACP) |
| SACE_5308 | 1730 | (pks7) | Missense (A795T) | Iterative type 1 polyketide synthase |
| Non-ribosomal peptide synthases and related biosynthetic proteins | ||||
| SACE_3015 | 1083 | (nrps4) | 1PM | Non-ribosomal peptide synthase |
| SACE_3016 | 334 | (nrps4) | Missense (V245F) | SyrP-like protein |
| SACE_3033 | 441 | Missense (R186C) | Lysine/ornithine N-monooxygenase | |
| SACE_3057 | 547 | Missense (A481T) | 2-polyprenyl-6-methoxyphenol hydroxylase | |
| SACE_4288 | 7259 | (nrpsl) | Missense (P6258L) | Non-ribosomal peptide synthase |
| Terpene synthases and related biosynthetic proteins | ||||
| SACE_3187 | 758 | (tpcl/geol) | Nonsense (W83*) | Terpene synthase metal-binding domain-containing protein |
| SACE_3978 | 466 | (tpc3/geo2) | Frameshift (+G 1040/1041) | Cyclic nucleotide-binding domain-containing protein (tpc3 gene cluster) |
aIPM, imperfect match
Abbreviations: CL thioester ligase; KSQ Initiating decarboxylase; KS ketosynthase; AT acyltransferase (A and P refer to specificity for acetate and propionate units respectively); ACP acyl carrier protein; KR ketoreductase; TE thioesterase; AMT aminotransferase; P450 cytochrome P450 hydroxylase; DH dehydratase; ER enoylreductase