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. 2012 May 23;7(5):e37859. doi: 10.1371/journal.pone.0037859

Figure 1. Distribution of MYT2 homologs in fungi and genetic complementation.

Figure 1

(A) Distribution of MYT2 in representative fungal species. The distribution image was constructed using the BLASTMatrix tool that is available on the Comparative Fungal Genomics Platform (http://cfgp.riceblast.snu.ac.kr/) [72]. (B) Phylogenetic tree of MYT2 homologs in several fungal species. The alignment was performed with ClustalW, and the MEGA program, version 4.0, was used to perform a 1,000-bootstrap phylogenetic analysis using the neighbor-joining method. Pi, Phytophthora infestans; Pr, P. ramorum; Ps, P. sojae; Af, Aspergillus fumigatus; An, A. nidulans; Ao, A. oryzae; Hc, Histoplasma capsulatum; Bc, Botrytis cinerea; Fo, Fusarium oxysporum; Fv, F. verticillioides; Mg, Magnaporthe grisea; Nc, Neurospora crassa; Pa, Podospora anserine; Ca, Candida albicans; Kl, Kluyveromyces lactis; Sc, Saccharomyces cerevisiae; Cc, Coprinus cinereus; Cn, Cryptococcus neoformans; Pc, Phanerochaete chrysosporium; nd, not detected. (C) Targeted deletion and complementation of MYT2. WT, G. zeae wild-type strain Z-3639; myt2, MYT2 deletion mutant; MYT2com, myt2-derived strain complemented with MYT2-GFP; A, AvaI; gen, geneticin resistance gene cassette; hyg, hygromycin B resistance gene cassette. Lane 1, Z-3639; lanes 2 and 3, MYT2 mutants; lanes 4 and 5, MYT2com mutants. The sizes of the DNA standards (kb) are indicated to the left of the blot.