Figure 1. Influence of peptidase inhibitors on the cytopathogenic effect of the cell-free filtrate.

LMH monolayer was incubated with cell-free filtrate with and without different peptidase inhibitors. (A) Mean lesion scores, (B) cytotoxicity of LMH cells, as assessed by Promega CellTiter 96® aqueous solution at different time points. Cell-free filtrate was obtained after 24 h of incubation of 10⁷ axenically grown protozoa cells obtained from T. gallinae clone 5895-C1/06, passage18. Absorbance values for Promega CellTiter 96® aqueous solution were recorded at 490 nm using ELISA reader.