Table 3. Results of the MS homology search for the protein Spot 5II.
| Protein Score | Peptide Score | Peptide Sequence | Matching Sequence | MS-Digest Index Nr. | Protein MW (Da)/pI | Accession Nr. | Species | Protein Name |
| 100 | 37 | NYWIVR | (K)NYWIVR(N) | 1635330 | 33644/6.2 | A2ET02 | T. vaginalis | Clan CA, family C1, cathepsin L-like cysteine peptidase |
| 63 | VNVVEGDEADLATK | (Y)VNVVEGDEKDLATK(V) | ||||||
| 100 | 37 | NYWIVR | (K)NYWIVR(N) | 2044283 | 33663/6.3 | B6CAS9 | T. vaginalis | Cytotoxic cysteine proteinase |
| 63 | VNVVEGDEADLATK | (Y)VNVVEGDEKDLATK(V) | ||||||
| 96 | 37 | NYWIVR | (K)NYWIVR(N) | 1626529 | 33768/7.5 | A2E0N7 | T. vaginalis | Clan CA, family C1, cathepsin L-like cysteine peptidase |
| 59 | VNVVEGDEADLATK | (Y)VNVAEGDEKDLATK(V) | ||||||
| 96 | 37 | NYWIVR | (K)NYWIVR(N) | 1641664 | 33796/6.9 | A2FD35 | T. vaginalis | Clan CA, family C1, cathepsin L-like cysteine peptidase |
| 59 | VNVVEGDEADLATK | (Y)VNVAEGDEKDLATK(V) | ||||||
| 96 | 37 | NYWIVR | (K)NYWIVR(N) | 2595891 | 32281/7.6 | Q27109 | T. vaginalis | Cysteine proteinase, putative (Fragment) |
| 59 | VNVVEGDEADLATK | (Y)VNVAEGDEKDLATK(V) | ||||||
| 96 | 37 | NYWIVR | (K)NYWIVR(N) | 2632915 | 33798/7.5 | Q49P75 | T. vaginalis | Cathepsin L-like cysteine proteinase |
| 59 | VNVVEGDEADLATK | (Y)VNVAEGDEKDLATK(V) | ||||||
| 94 | 37 | NYWIVR | (K)NYWIVR(N) | 1621053 | 33733/7.0 | A2DJ07 | T. vaginalis | Clan CA, family C1, cathepsin L-like cysteine peptidase |
| 57 | VNVVEGDEADLATK | (Y)INVVEGDEKDLAAK(V) | ||||||
| 94 | 37 | NYWIVR | (K)NYWIVR(N) | 1636066 | 34189/6.8 | A2EVA2 | T. vaginalis | Clan CA, family C1, cathepsin L-like cysteine peptidase |
| 57 | VNVVEGDEADLATK | (Y)INVVEGDEKDLAAK(V) | ||||||
| 92 | 31 | NYWIVR | (T)KYWIVR(N) | 1638461 | 33928/5.1 | A2F2X0 | T. vaginalis | Clan CA, family C1, cathepsin L-like cysteine peptidase |
| 61 | VNVVEGDEADLATK | (Y)INVVEGDENDLATK(I) | ||||||
| 81 | 37 | NYWIVR | (K)NYWIVR(N) | 1650317 | 31394/7.9 | A2G6Q5 | T. vaginalis | Clan CA, family C1, cathepsin L or K-like cysteine peptidase |
| 44 | VNVVEGDEADLATK | (Y)VTVNEGDEKDLAKK(V) | ||||||
| 81 | 37 | NYWIVR | (K)NYWIVR(N) | 2761380 | 31250/6.9 | Q6UEJ4 | T. vaginalis | Papain-like cysteine proteinase |
| 44 | VNVVEGDEADLATK | (Y)VTVNEGDEKDLAKK(V) |
The top 10 matches from the search are displayed. Following parameters were used for search: Taxonomy search in Trichomonas vaginalis; Database searched: UniProtKB.2011.01.11; Mass Tolerance: 0.5 Da; Digest Used: No enzyme; Max. # Missed Cleavages: 1; Min matches: 1; Score matrix: BLOSUM62; List of Peptide Sequences: 1 VNVVEGDEADLAT [Q|K] 4, 2 NYW [I|L]VR 1 (number left to the peptide sequence is used as the name of the peptide, whereas the number right to the peptide sequence refers to the maximal number of allowed mismatches). Mismatched amino acids are shown in bold.