Figure 4. Normal cellular influx and the generation of antigen-specific T cells in the absence of IDO-1 in vivo.
WT and IDO-1−/− mice were infected with M. tuberculosis (100 CFU). Single cell suspensions were prepared from infected lungs and analyzed by flow cytometry for (A) CD4+, (B) CD8+ cells, and the percentages of these cells with an activated CD44hi/CD62lo phenotype ((C) CD4+, (D) CD8+). (E) Numbers of IFN-γ producing cells determined by ELISpot for lung cells cultured at 1×105 cells/well with M. tuberculosis CFP overnight. Data represent the mean +/− SD of 5 mice from one representative of two experiments.