Table 1. Development of vitrified-warmed germinal vesicle (GV)-stage oocytes with or without antifreeze protein (AFP; 500 ng/mL) supplementation in vitrification media (seven replicates).
| AFP-treated | Untreated | P | |
| Initiated GV oocyte | 171 | 178 | |
| Survived | 166 (97.1%) | 162 (91.0%) | 0.017 |
| Matured | 129 (77.7%) | 126 (77.8%) | NS |
| Cleaved | 118 (91.5%) | 88 (69.8%) | <0.01 |
| Blastocyst (per cleaved embryo) | 96 (81.4%) | 59 (67.1%) | 0.019 |
| Blastocyst (per initiated GV) | 96 (56.1%) | 59 (33.2%) | <0.01 |
| Blastomere count per blastocyst | 78.2±11.8a | 67.8±11.3b | <0.01 |
| No. of caspase-positive blastomeres | 285/3,987 (7.1%) | 363/3,188 (11.4%) | <0.01 |
| No. of trophectoderms perblastocyst | 69.6±11.8c | 54.0±5.3d | <0.01 |
| No. of inner cell masses perblastocyst | 13.9±2.2c | 11.4±1.9d | <0.01 |
Mean±SD.
Number of blastocysts tested: a = 51, b = 47, c = 23 d = 22.