Figure 5. Expression analysis of DIL1 gene.

A. DIL1 mRNA levels in V3 leaves in wild type and mutant plants, as determined by RT-PCR with 30 cycles for DIL1 (Upper), with the actin gene as the control (Lower). B-D. RNA in situ hybridization on tissue sections from A619 wild type vegetative apices, showing expression of DIL1 in the leaf primordia and the P₀ region of the meristem (B and C). Hybridization signal was not observed with control, sense probes (D).