Skip to main content
. 2012 May 23;7(5):e37632. doi: 10.1371/journal.pone.0037632

Figure 2. Kinetic analysis of the effect of AIEP on the binding of MgATP and IAA by GH3-1 and GH3-6.

Figure 2

The activity of GH3-1 (A) and GH3-6 (B) was determined over a concentration range of MgATP (1 mM IAA, 1 mM Asp) by quantifying the formation of IAA-Asp after 10 min reaction time using LC-ESI-MS/MS. The same analysis was performed over a concentration range of IAA (3 mM MgATP, 1 mM Asp) using either GH3-1 (C) or GH3-6 (D). The inhibitor concentration in the reaction mix was 0 µM (•), 0.1 µM (○), 1 µM (▴), 5 µM (Δ), 10 µM (▪), or 50 µM (□). The plotted initial velocities were fitted to the Michaelis-Menten equation using non-linear regression (SigmaPlot 11.0). All data represent mean ± standard error of the mean (n = 3).