Figure 4. AIEP inhibits GH3-1 and GH3-6 in a competitive manner regarding both substrates – MgATP and IAA.

Reciprocal initial velocities (Fig. 2) for two substrate conentrations were replotted against inhibitor concentrations. (A) Dixon plot for GH3-1 and (B) GH3-6 with varying concentrations of inhibitor and 100 µM (◊) and 1000 µM (○) MgATP and for (C) GH3-1 and (D) GH3-6 with varying concentrations of inhibitor and 10 µM (□) and 100 µM (Δ) IAA. All data represent mean ± standard error of the mean (n = 3).