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. 2012 May 23;7(5):e37513. doi: 10.1371/journal.pone.0037513

Figure 2. Circulating CD4+ CD25+ CD127− Tregs are decreased significantly in GV patients.

Figure 2

(A) Human PBMCs were labeled with lymphocyte-specific antibodies (CD4, CD25 and CD127). Representative flow data showing the gating strategy and percent of natural Treg cells within the CD4+ T cell population are given for one patient from each of the 3 studied groups. (B) A negative correlation was found between the frequency of CD4+ natural Treg cells and the expression of IFN-γ (r = −0.512, P = 0.001), GrB (r = −0.524, P = 0.001) or Perforin (r = −0.585, P<0.001) from CD3+ CTLs in patients with progressive GV (n = 38). (C) The results of the flow cytometric analysis of natural Treg cells in patients with progressive GV (n = 38), stable GV (n = 12) and control subjects (n = 20) are shown. * = P<0.01, ** = P<0.001. (D) Longitudinal monitoring of natural Treg cells in 6 patients. The percentage of CD4+ natural Treg cells was measured twice: once during a vitiligo flare and once following resolution after treatment. * = P<0.05.