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. Author manuscript; available in PMC: 2013 May 29.
Published in final edited form as: Chembiochem. 2012 Apr 24;13(8):1191–1198. doi: 10.1002/cbic.201200065

Figure 5. Transferrin uptake in GA treated cells.

Figure 5

HeLa cells were treated with DMSO (top row) or GA (bottom row) for 1h and then are incubated at 4 °C for 5 min. Transferrin (Tf-488) was added at 4 °C for 45 min to allow binding to the Transferrin receptor at the plasma membrane (a, d). To allow Tf-488 internalization within the endocytic compartment, the cells are transferred at 37 °C for 5 min or 10 min in the absence (b, c) or presence (e, f) of GA. Tf-488 is in green; mitochondria are in red; nuclei are in blue.