. Author manuscript; available in PMC: 2013 May 1.

Published in final edited form as: Mech Ageing Dev. 2012 Feb 24;133(5):234–245. doi: 10.1016/j.mad.2012.02.001

Table 1. Longevity of 45 EP lines induced by da-Gal4.

Forty-five isogenic EP lines, in which an EP is inserted in the same orientation of normal transcription, were crossed with either isogenic CS10 or isogenic da-Gal4. Lifespans of their male progeny were measured at 25°C. The target genes of these EP lines are categorized into 8 groups, based on their functions. Fifteen EP lines that as da-Gal4^CS10 >EP^CS10 presented significantly longer mean lifespan than EP^CS10/+^CS10, da-Gal4^CS10/+^CS10 (33.6 d) and +^CS10/+^CS10 (33.4 d) were selected as longevity candidates (bold type; p < 0.001 by Log-Lank test). Five EP lines (GX193, GX7554, GX5503-1, GX8277, and GX6548) were lethal when induced by da-Gal4. Human homologs of these genes are indicated in the parentheses. Mean lifespan (MLS), maximum lifespan (MaxLS, day when the final survivor died), and increased lifespan (ILS, ratio of da-Gal4^CS10 >EP^CS10 to EP^CS10/+^CS10) are presented with the number (n) of flies tested.

EP line	Gene	Description (Human Homolog)	/+^CS10			/da- Gal4^CS10			ILS	Log-Rank Probability
			/+^CS10			/da- Gal4^CS10				+^CS10 /+^CS10	Gal4^CS10 /+^CS10	EP^CS10 /+^CS10

			MLS	MaxLS	(n)	MLS	MaxLS	(n)		vs. da -Gal4^CS10 >EP^CS10
Transcription & translation
GX193	CG10209	DNA binding	39.4	63.0	(361)		lethal		–	–	–	–
GX2970	*kis*	DNA helicase (CDH7)	37.8	62.0	(357)	51.4	76.0	(382)	1.36	< 0.001	< 0.001	< 0.001
GX6561	*kis*	DNA helicase (CDH7)	36.6	59.0	(348)	45.3	76.0	(345)	1.24	< 0.001	< 0.001	< 0.001
GX7554	dan	Transcription factor	42.7	71.0	(360)		lethal		–	–	–	–
GX8261	gug	Histone deacetylase	38.7	62.0	(385)	37.7	80.0	(358)	0.97	< 0.001	< 0.001	0.293
GX8304	*Sin3A*	Transcription repressor (SIN3A)	50.5	78.0	(251)	57.2	80.0	(237)	1.13	< 0.001	< 0.001	< 0.001
GX8403	RpL13A	Ribosomal protein L13 (RPL13A)	44.0	66.0	(172)	44.2	66.0	(220)	1.00	< 0.001	< 0.001	0.687
GX21702	Rat1	5’-3’ exoribonuclease (XRN2)	39.4	62.0	(285)	34.8	56.0	(323)	0.88	0.051	0.320	< 0.001
GX21702-1	sm	Heterogeneous nuclear ribonucleoprotein L (HNRNPL)	40.4	62.0	(383)	52.1	72.0	(257)	1.29	< 0.001	< 0.001	< 0.001
GX25754	CG4901	ATP-dependent RNA helicase (DHX33)	37.5	70.0	(613)	34.6	63.0	(594)	0.92	0.008	0.034	< 0.001
GX31631	Thor	Eukaryotic initiation factor 4E binding	46.2	75.0	(593)	46.7	77.0	(567)	1.01	< 0.001	< 0.001	0.567
GX46252	mam	Transcription coactivator	43.3	70.0	(611)	41.8	72.0	(586)	0.97	< 0.001	< 0.001	0.107
EP(2)2559	CG3927	RNA binding	31.4	60.0	(559)	34.9	65.0	(602)	1.11	0.013	0.080	< 0.001
Cell cycle
GX822	CycG	Cyclin-dependent protein kinase regulator (CCNG2)	40.2	69.0	(577)	38.1	73.0	(549)	0.95	< 0.001	< 0.001	0.420
GX11242	CycB3	Cyclin B3	43.6	68.0	(362)	39.4	64.0	(327)	0.90	< 0.001	< 0.001	< 0.001
Signal transduction
GX4499	*ImpL2*	Ecdysone-inducible gene L2	39.8	60.0	(391)	45.7	74.0	(385)	1.15	< 0.001	< 0.001	< 0.001
GX47280-1	*SIFR*	Neuropeptide receptor (NPFFR2)	38.4	66.0	(364)	47.1	68.0	(381)	1.23	< 0.001	< 0.001	< 0.001
GX5503-1	Rapgap1	Ras GTPase activator (RAP1GAP)	36.3	58.0	(350)		lethal		–	–	–	–
GX6589	cv-2	Cysteine-rich domain (BMPER)	34.6	60.0	(388)	34.8	58.0	(377)	1.01	0.028	0.082	0.548
GX8277	gom	Calcium ion binding	37.1	56.0	(317)		lethal		–	–	–	–
GX8630	Lrch	Leucine-rich-repeats and calponin homology domain protein (LRCH2)	39.4	69.0	(631)	40.0	65.0	(539)	1.02	< 0.001	< 0.001	0.673
GX8689	vimar	Ral GTPase binding (RAP1GDS1)	33.6	66.0	(330)	33.6	60.0	(371)	1.00	0.597	0.977	0.474
GX16168	*CalpA*	Calcium-dependent cysteine-type endopeptidase (CAPN9)	37.5	64.0	(368)	42.0	60.0	(347)	1.12	< 0.001	< 0.001	< 0.001
EP(2)2612	*CG8155*	Rab GTPase activator (TBC1D25)	39.7	76.0	(369)	45.6	74.0	(294)	1.15	< 0.001	< 0.001	< 0.001
Cellular component movement
GX48290	*Dlc90F*	Dynein intermediate chain binding (DYNLT1)	36.5	56.0	(197)	39.8	64.0	(224)	1.09	< 0.001	< 0.001	0.002
Cellular metabolism
GX1008	nemy	Carbon-monoxide oxygenase	33.0	56.0	(375)	35.3	59.0	(296)	1.07	0.017	0.072	0.035
GX1008-1	CG42708	Glutaminase	40.4	66.0	(317)	40.3	61.0	(342)	1.00	< 0.001	< 0.001	0.731
GX4385	CG13890	Dodecenoyl-CoA delta-isomerase	36.8	60.0	(626)	36.5	63.0	(546)	0.99	< 0.001	< 0.001	0.297
GX8295	arf51F	NAD(P)⁺-protein-arginine	35.2	62.0	(280)	33.9	58.0	(336)	0.96	0.032	0.518	0.111
GX8331	*CG33138*	1,4-alpha-glucan branching enzyme (GBE1)	41.9	72.0	(340)	46.4	72.0	(354)	1.11	< 0.001	< 0.001	< 0.001
GX47642-1	*CG10383*	Hydrolase (SERAC1)	42.0	68.0	(372)	47.2	74.0	(285)	1.12	< 0.001	< 0.001	< 0.001
GX56643	eco	Acetyltransferase	47.3	72.0	(223)	36.8	66.0	(204)	0.78	< 0.001	< 0.001	< 0.001
GX62810	fabp	Fatty acid binding	41.9	71.0	(593)	42.1	72.0	(585)	1.00	< 0.001	< 0.001	0.219
EP(3)1250	*men*	NADP-dependent malate dehydrogenase (ME3)	39.5	72.0	(350)	52.4	76.0	(374)	1.33	< 0.001	< 0.001	< 0.001
EP(2)2086	*CG30427*	Oxidoreductase	38.1	66.0	(380)	44.9	66.0	(464)	1.18	< 0.001	< 0.001	< 0.001
Transport
GX26268	Atpα	Na pump α subunit (ATP1A3)	52.2	78.0	(619)	49.9	77.0	(597)	0.96	< 0.001	< 0.001	0.012
EP(3)3232	drip	Water channel (AQP4)	48.0	76.0	(330)	42.3	74.0	(344)	0.88	< 0.001	< 0.001	< 0.001
Immunity
GX5503	*PGRP-LF*	Peptidoglycan recognition protein LF (PGLYRP3)	31.3	51.0	(356)	35.5	63.0	(351)	1.13	< 0.001	0.002	< 0.001
GX6548	PGRP-LC	Peptidoglycan recognition protein LC	44.3	70.0	(372)		lethal		–	–	–	–
Others
GX1042	CG42268	Unknown	36.3	60.0	(345)	35.6	63.0	(362)	0.98	< 0.001	< 0.001	0.606
GX2970-1	*CG42663*	Unknown	36.0	60.0	(368)	42.5	64.0	(356)	1.18	< 0.001	< 0.001	< 0.001
GX3571	Mbs	Myosin phosphatase (PPP1R12A)	55.9	83.0	(387)	47.2	78.0	(350)	0.84	< 0.001	< 0.001	< 0.001
GX8400	CG5861	Transmembrane protein (TMEM147)	40.3	66.0	(347)	37.3	62.0	(324)	0.93	< 0.001	< 0.001	< 0.001
GX47642	*CG10916*	Zinc ion binding	32.0	62.0	(361)	40.8	78.0	(293)	1.28	< 0.001	< 0.001	< 0.001
GX62808	CG5807	Limb region 1 homolog-like (LMBR1L)	47.5	75.0	(596)	42.6	70.0	(572)	0.90	< 0.001	< 0.001	< 0.001