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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: J Cell Physiol. 2012 Oct;227(10):3446–3456. doi: 10.1002/jcp.24045

FIG.2. Runx2 and Col10α1 mRNA is upregulated in transgenic mice at E17.5 and P1 stages.

FIG.2

(A). Immunohistochemistry analysis was conducted on TG mouse hind limb sections with or without anti-Flag antibody. Brown staining showing Flag expression was primarily restricted to the pre-hypertrophic and hypertrophic chondrocytes of proximal tibia (right panel, red arrows). Left panel shows no antibody control. TG: Transgenic. HZ: hypertrophic zone. (B). Relative mRNA transcripts of Col10a1 and Runx2 were examined using total RNAs prepared from whole hind limbs at E17.5 stage. Runx2 showed two-fold upregulation in transgenic (TG) mice compared to their wild-type (WT) littermates (WT vs. TG: 1.00 ± 0.20 vs. 2.10 ± 0.36, n = 6, t = 2.69, df = 10, p = 0.02). Meanwhile, Col10a1 expression was also increased in TG mice (WT vs. TG: 1.00 ± 0.32 vs. 2.89 ± 0.77, n = 5, t = 2.27, df = 8, p = 0.05). (C). As illustrated, both Runx2 and Col10a1 showed similar increased level of mRNA transcripts in TG mice at P1 stage (Runx2, WT vs. TG: 1.00 ± 0.15 vs. 2.25 ± 0.43, n = 6, t = 2.78, df = 10, p = 0.02; Col10α1, WT vs. TG: 1.00 ± 0.26 vs. 3.11 ± 0.63, n = 5-6, t = 3.30, df = 9, p = 0.009). (D). Runx2 and Col10a1 also showed upregulation in transgenic mice using total RNAs prepared from hypertrophic zone-enriched tissues at P1 stag (Runx2, WT vs. TG: 1.00 ± 0.13 vs. 1.94 ± 0.28, n = 4-8, t = 2.04, df = 10, p = 0.043; Col10α1, WT vs. TG: 1.00 ± 0.19 vs. 2.59 ± 0.43, n = 4-8, t = 2.50, df = 10, p = 0.031). TG: transgenic; WT: wild-type littermates. Bars denote means ± SE for Runx2 and Col10α1 expression. “*”: p < 0.05; “**”: p < 0.01.