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. Author manuscript; available in PMC: 2013 May 29.
Published in final edited form as: Chembiochem. 2012 Apr 19;13(8):1112–1115. doi: 10.1002/cbic.201200091

Figure 1.

Figure 1

N6pA is incorporated by pol I in cells. (a) Structure of N6pA and N6pATP. (b) Cells were incubated for 12 hours in media containing the indicated concentrations of N6pA. After fixation and permeabilization, rhodoamine-azide was conjugated via click chemistry. Top panels are rhodamine signal and the bottom panels are nuclei counterstained with TO-PRO-3. (c) Cells were incubated with 1 mM N6pA in the presence of actinomycin D or hydroxyurea as indicated; fluorescent assay performed as in (b).