Figure 3.

N⁶pA is incorporated into pol II and pol III transcripts in vivo. RNA harvested from cells treated with DMSO, 200 µM EU, or 200 µM N⁶pA for 12 hr was subject to click chemistry with biotin-azide and selected on a streptavidin column. 10% of the input and 100% of the bound RNA was analyzed by semi-quantitative RT-PCR using primers specific for the histone H2A mRNA or the 7SK small noncoding RNA. Negative controls lacking reverse transcriptase are also shown (-RT).