Table 3.
Transcriptional fusions to uidA confirm that PodJ1 affects expression of diverse genes.
| Gene | LB | PYE | ||||
|---|---|---|---|---|---|---|
| Rm1021 | ΔpodJ1 | Ratio | Rm1021 | ΔpodJ1 | Ratio | |
| Increased expression in ΔpodJ1 | ||||||
| SMb20838 | 0.5 ± 0.2 | 1.3 ± 0.4 | 252% | 0.4 ± 0.0 | 2.7 ± 0.9 | 728% |
| SMb20999 (bacA) | 11.0 ± 2.3 | 15.2 ± 5.1 | 138% | 6.9 ± 1.8 | 7.2 ± 2.3 | 104% |
| SMb21522 (minE) | 15.5 ± 2.5 | 21.3 ± 1.9 | 138% | 14.8 ± 2.7 | 19.7 ± 3.7 | 133% |
| SMc02389 | 0.8 ± 0.2 | 2.2 ± 0.5 | 280% | 1.4 ± 0.1 | 3.3 ± 0.6 | 229% |
| SMc03108 | 21.6 ± 3.7 | 47.0 ± 11.1 | 217% | 25.2 ± 3.6 | 33.8 ± 3.5 | 134% |
| SMc04296 (ftsZ2) | 24.6 ± 0.9 | 38.1 ± 3.7 | 155% | 39.5 ± 6.3 | 46.7 ± 8.3 | 118% |
| Decreased expression in ΔpodJ1 | ||||||
| SMc00887 | 7.9 ± 0.9 | 2.0 ± 0.5 | 25% | 32.4 ± 5.2 | 20.7 ± 2.9 | 64% |
| SMc00888 | 20.1 ± 1.6 | 5.2 ± 1.2 | 26% | 90.4 ± 16.0 | 55.5 ± 5.2 | 61% |
| SMc00975 (mcpU) | 5.6 ± 1.2 | 1.3 ± 0.2 | 23% | 58.5 ± 8.5 | 37.8 ± 6.4 | 65% |
| SMc01104 (mcpX) | 0.9 ± 0.3 | 0.1 ± 0.0 | 14% | 8.7 ± 2.4 | 2.1 ± 0.4 | 23% |
| SMc03040 (flaC) | 5.6 ± 0.7 | 1.5 ± 0.2 | 26% | 10.0 ± 1.6 | 8.0 ± 1.4 | 80% |
| SMc04011 (tacA) | 20.8 ± 4.8 | 0.7 ± 0.2 | 3% | 42.1 ± 4.6 | 1.7 ± 0.3 | 4% |
| SMc04114 (pilA1) | 0.8 ± 0.1 | 0.2 ± 0.0 | 19% | 8.4 ± 1.8 | 0.8 ± 0.2 | 10% |
Beta-glucuronidase activity (± standard deviation, in Miller units) of strains grown in LB or PYE was measured as described in Experimental Procedures. Ratio equals the enzymatic activity in the mutant divided by that in the Rm1021 wild-type. Expression is significantly different between the two strains in all comparisons (p < 0.01), except for SMb20999 (bacA) in PYE.