Table 5.

Phenotypic characterization of ΔpodJ1 suppressors.

Genotypea	Motilityb	PYE	LB	LBLS	Deoxych	Calcofluorc
Rm1021 (wild-type)	100%	+++	+++	+++	+++	100%
ΔSMc00067	109%	+++	+++	+++	+++	70% ± 11%	**
ΔSMc00122	103%	+++	+++	+++	++	73% ± 30%	**
ΔSMc00457	106%	+++	+++	+++	++	79% ± 28%	**
ΔSMc02219	110%	+++	+++	+++	++	78% ± 28%	*
ΔSMc02555	109%	+++	+++	+++	+++	81% ± 31%	*
ΔSMc03855	109%	+++	+++	+++	+++	91% ± 14%	*
ΔSMc03872	108%	+++	+++	+++	++	71% ± 19%	**
SMc03996::Tn5	102%	++	++	+++	+	48% ± 10%	**
pleCSm::Tn5	101%	+++	+++	+++	+++	102% ± 7%
Rm1021 / P-pleCSm	99%	+++	+++	+++	+++	110% ± 14%
ΔpodJ1	36%	+++	+++	−	−	163% ± 48%	**
ΔpodJ1 ΔSMc00067	50%	+++	+++	+++	++	76% ± 12%	^^
ΔpodJ1 ΔSMc00122	44%	+++	+++	++	−	148% ± 59%	^^
ΔpodJ1 ΔSMc00457	45%	+++	+++	++	−	133% ± 44%	^
ΔpodJ1 ΔSMc02219	42%	+++	+++	++	−	155% ± 61%	^^
ΔpodJ1 ΔSMc02555	45%	+++	+++	++	−	134% ± 47%	^^
ΔpodJ1 ΔSMc03855	41%	+++	+++	+	−	106% ± 32%	^^
ΔpodJ1 ΔSMc03872	45%	++	+++	++	+/−	93% ± 38%	^^
ΔpodJ1SMc03996::Tn5	62%	++	++	+	−	70% ± 18%	^^
ΔpodJ1pleCSm::Tn5	59%	+++	+++	+++	+	158% ± 29%
ΔpodJ1 / P-pleCSm	50%	+++	+++	++	+	158% ± 34%

The ability of strains to grow on various media was compared to that of wild-type Rm1021: +++ indicates the best growth, while − indicates the worst or no growth. Deoxych indicates LB plates containing 0.1% sodium deoxycholate.

^aP-pleC_Sm represents P_lac-P_pleC-pleC_Sm, which allows moderate overexpression of pleC_Sm from pJC466.

^bThe diameters of motility swarms on soft-agar plates were compared to those of the wild-type at least three independent times and the resultant ratios averaged. Standard deviation for each average was less than 10%. The average diameter of the wild-type swarm after 3 days in PYE was 17 mm.

^cRelative fluorescence intensity (± standard deviation) on plates containing Calcofluor is the average of at least 12 different dilution spots; * if 0.01 < p ≤ 0.05, ** if p ≤ 0.01, when compared to wild-type; ^ if 0.01 < p ≤ 0.05, ^^ if p ≤ 0.01, when compared to ΔpodJ1.