Table 6.
S. meliloti strains used in this study
| Strains | Relevant genetic markers, features, and/or description a | Construction, source, or reference b |
|---|---|---|
| Rm1021 | SU47 derivative SmR (progenitor of strains listed below) | (Meade et al., 1982) |
| Rm7096 | exoS96::Tn5 | (Doherty et al., 1988) |
| Rm7210 | exoY::Tn5 | (Zhan et al., 1990) |
| R5D6 | lpsB::Tn5–110 | (Griffitts and Long, 2008) |
| MB669 | flaC’-uidA | (Gibson et al., 2007) |
| VO2119 | ΔbacA::aadA | (Oke and Long, 1999) |
| VO2196 | PbacA-uidA (SMb20999::uidA) | (Starker et al., 2006) |
| JOE2420 | podJ1(SMc02230)::pJC358 | Mate pJC358 into Rm1021, select for NmR |
| JOE2422 | podJ2(SMc02231)::pJC359 | Mate pJC359 into Rm1021, select for NmR |
| JOE2461 | pleCSm::pJC364 (pleCSm-egfp) | Mate pJC364 into Rm1021, select for NmR |
| JOE2553 | ΔpodJ1 (ΔSMc02230) | Allelic replacement using pJC376 |
| JOE2608 | ΔpodJ1::Ω | Allelic replacement using pJC380 |
| JOE2612 | podJ2(SMc02231)…Ω (SpR wild-type strain, with antibiotic marker inserted after podJ2) | Allelic replacement using pJC382 |
| JOE2968 | flaC’-uidA | Rm1021 × Φ(MB669), select for SpR |
| JOE2970 | ΔpodJ1 flaC’-uidA | JOE2553 × Φ(MB669), select for SpR |
| JOE3241 | ΔpodJ1::Ω PbacA-uidA | VO2196 × Φ(JOE2608), select for SpR |
| JOE3243 | ΔSMc00122 | Allelic replacement using pJC436 |
| JOE3245 | ΔSMc03855 | Allelic replacement using pJC438 |
| JOE3247 | ΔSMc02219 | Allelic replacement using pJC439 |
| JOE3249 | ΔSMc03872 | Allelic replacement using pJC440 |
| JOE3269 | pleCSm::pJC443 (pleCSm-mCherry) | Mate pJC443 into Rm1021, select for SpR |
| JOE3287 | ΔpodJ1::Ω ΔSMc00122 | JOE3243 × Φ(JOE2608), select for SpR |
| JOE3289 | ΔpodJ1::Ω ΔSMc03855 | JOE3245 × Φ(JOE2608), select for SpR |
| JOE3291 | ΔpodJ1::Ω ΔSMc02219 | JOE3247 × Φ(JOE2608), select for SpR |
| JOE3293 | ΔpodJ1::Ω ΔSMc03872 | JOE3249 × Φ(JOE2608), select for SpR |
| JOE3306 | PbacA-uidA | Rm1021 × Φ(VO2196), select for NmR |
| JOE3308 | ΔpodJ1 PbacA-uidA | JOE2553 × Φ(VO2196), select for NmR |
| JOE3328 | ΔSMc00457 | Allelic replacement using pJC448 |
| JOE3330 | ΔSMc02555 | Allelic replacement using pJC449 |
| JOE3366 | ΔSMc00067 | Allelic replacement using pJC454 |
| JOE3393 | ΔpodJ1 / pJC456 | Mate pJC456 into JOE2553, select for TcR |
| JOE3396 | ΔpodJ1::Ω ΔSMc00457 | JOE3396 × Φ(JOE2608), select for SpR |
| JOE3398 | ΔpodJ1::Ω ΔSMc02555 | JOE3330 × Φ(JOE2608), select for SpR |
| JOE3400 | ΔpodJ1::Ω ΔSMc00067 | JOE3366 × Φ(JOE2608), select for SpR |
| JOE3402 | ΔpleCSm::Ω (ΔSMc02369::Ω) / pJC456 | Allelic replacement using pJC452 |
| JOE3446 | ΔpodJ1 ΔpleCSm::Ω / pJC456 | JOE3393 × Φ(JOE3402), select for SpR |
| JOE3465 | pleCSm::Tn5–110 (SMc02369::Tn5–110) | Rm1021 × Φ(CSN215), select for NmR |
| JOE3467 | SMc03996::Tn5–110 | Rm1021 × Φ(CSN136), select for NmR |
| JOE3469 | ΔpodJ1pleCSm::Tn5–110 | JOE2553 × Φ(CSN215), select for NmR |
| JOE3470 | ΔpodJ1 SMc03996::Tn5–110 | JOE2553 × Φ(CSN136), select for NmR |
| JOE3593 | Rm1021 / pJC476 (Ptau-pleCSm) | Mate pJC476 into Rm1021, select for TcR |
| JOE3601 | pleCSm::pJC468 (Ptau-pleCSm) | Mate pJC468 into Rm1021, select for NmR |
| JOE3608 | ΔpleCSm::Ω / pJC476 (Ptau-pleCSm) | JOE3593 × Φ(JOE3402), select for SpR |
| JOE3626 | ΔpodJ1 exoY::Tn5 | JOE2553 × Φ(Rm7210), select for NmR |
| JOE3628 | ΔpodJ1 exoS96::Tn5 | JOE2553 × Φ(Rm7096), select for NmR |
| JOE3684 | ΔpodJ2 (ΔSMc02231) | Allelic replacement using pJC489 |
| JOE3687 | ΔpodJSm (ΔSMc02230–SMc02231) | Allelic replacement using pJC490 |
| JOE3762 | podJ1::pJC502 (podJ1-mCherry) | Mate pJC502 into Rm1021, select for SpR |
| JOE3764 | podJ2::pJC501 (podJ2-mCherry) | Mate pJC501 into Rm1021, select for SpR |
| CSN015 | ΔpodJ1 SMc00067::Tn5–110 (1107356 Rev) | Suppressor selection |
| CSN016 | ΔpodJ1 SMc00067::Tn5–110 (1107487 Rev) | Suppressor selection |
| CSN114 | ΔpodJ1 SMc00122::Tn5–110 (1102099 Rev) | Suppressor selection |
| CSN116 | ΔpodJ1 SMc02219::Tn5–110 (581597 Rev) | Suppressor selection |
| CSN121 | ΔpodJ1 SMc03855::Tn5–110 (3520402 Rev) | Suppressor selection |
| CSN123 | ΔpodJ1 SMc02219::Tn5–110 (582227 For) | Suppressor selection |
| CSN125 | ΔpodJ1 SMc02219::Tn5–110 (581779 For) | Suppressor selection |
| CSN130 | ΔpodJ1 SMc02219::Tn5–110 (582268 For) | Suppressor selection |
| CSN132 | ΔpodJ1 SMc02555::Tn5–110 (1206913 For) | Suppressor selection |
| CSN133 | ΔpodJ1 SMc00457::Tn5–110 (1100379 Rev) | Suppressor selection |
| CSN134 | ΔpodJ1 SMc03872::Tn5–110 (3537402 Rev) | Suppressor selection |
| CSN135 | ΔpodJ1 SMc00122::Tn5–110 (1102467 Rev) | Suppressor selection |
| CSN136 | ΔpodJ1 SMc03996::Tn5–110 (2994619 Rev) | Suppressor selection |
| CSN197 | ΔpodJ1 SMc02555::Tn5–110 (1206837 For) | Suppressor selection |
| CSN214 | ΔpodJ1 SMc02555::Tn5–110 (1206923 For) | Suppressor selection |
| CSN215 | ΔpodJ1 SMc02369::Tn5–110 (1121237 For) | Suppressor selection |
a
Because some Tn5–110 insertions were isolated multiple times, only representative isolates are listed. Numbers in parenthesis after Tn5–110 indicate the insertion position (see Table 1)
b
Φ indicates generalized transduction, as mediated by bacteriophage ΦN3. For example, JOE3366 × Φ(JOE2608) means that a bacteriophage lysate made from JOE2608 was used to infect JOE3366.