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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Hippocampus. 2011 Nov 10;22(6):1467–1480. doi: 10.1002/hipo.20986

Figure 7.

Figure 7

Localization of SK2 channels along the surface of CA1 pyramidal cells using the SDS-digested freeze-fracture replica labeling (SDS-FRL) technique. Freeze-fracture replicas prepared from mouse hippocampus were labeled with 5-nm (small black dots, arrows) and 10-nm (bold black dots) immunoparticles to detect PSD-95 and SK2, respectively. SK2 is immunolabeled with an antibody directed against an epitope at the intracellular C-terminus, so the two proteins can be detected at the P-face of the plasma membrane. The E-face is free of any immunolabeling. (A-B) Clusters of immunoparticles for SK2 were detected in dendritic spines in the proximal radiatum (panel A) and distal radiatum (panel B) in close proximity or intermingled with PSD-95. (C) Antibody specificity is controlled and confirmed in replicas of SK2 null mice that were free of any immunolabeling. Scale bars: A-C, 0.2 μm.