Figure 4. Temporal effect of PI3K inhibition on mEH protein.
PND4 F344 rat ovaries were cultured in media containing vehicle control (Agency), ± 20 μM LY294002 for 4 or 6 d. Total protein was isolated and Western blotting was performed to detect mEH protein. (A) Representative Western blot is shown on day 4; Control = C; LY294002 = L. (B) Values are expressed as a percentage of control mean ± SE; n=3 (10 ovaries per pool). * P < 0.05; different from control (Adapted from Bhattacharya et al., 2012 with copyright permission).
