Table 3.
Degradation rates (k) and half-lives (t 1/2) of EMs incubated with APM alone and in the presence of inhibitors
| Inhibitor | APM | |||
|---|---|---|---|---|
| EM-1 | EM-2 | |||
| 100 × k (1/min) | t 1/2 (min) | 100 × k (1/min) | t 1/2 (min) | |
| Without inhibitor | 3.51 ± 0.09 | 19.7 ± 0.50 | 2.96 ± 0.12 | 23.3 ± 0.98 |
| Actinonin | 1.88 ± 0.09 | 36.8 ± 2.10*** | 1.50 ± 0.05 | 46.3 ± 1.16** |
| Tyr-Pro-Ala-NH2 (EMDB-2) | 1.63 ± 0.06 | 42.3 ± 1.89*** | 1.28 ± 0.04 | 53.9 ± 1.53*** |
| Tyr-Pro-Ala-OH (EMDB-3) | 1.58 ± 0.05 | 43.7 ± 1.73*** | 1.44 ± 0.07 | 47.9 ± 2.14*** |
** P < 0.01, *** P < 0.001 as compared to respective EM incubated in the absence of inhibitor by using one-way ANOVA followed by Student–Newman–Keul’s test