Figure 5.

Diabetic rats demonstrate increased levels of anti-LC3 and anti-mitochondria (AMA) immunoreactivity that colocalize in dorsal root ganglion (DRG) neurons. (A) IHC detection of LC3 and colocalization with the mitochondrial AMA signal in DRG neurons from control and diabetic rats. (B) IHC detection of Beclin 1, LC3 and their colocalization in DRG neurons of normal, control and diabetic rats. (C) Representative immunoblot of mitochondrial (AMA) in DRG homogenates from normal (control) and diabetic rats. (D) Histogram of densitometric scannings of immunoblots of mitochondria (AMA) in DRG homogenates from control and diabetic rat DRG neurons. The normal control was set to 100%. Scale bar, 50 µm. *p < 0.05. Adapted from reference ¹⁰⁹, with permission.