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. 2011 Nov 1;7(11):1359–1370. doi: 10.4161/auto.7.11.17660

Figure 2.

Figure 2

B. cepacia resides in an autophagosome-like compartment in macrophages. (A and B) confocal microscopy for wild-type (WT) murine BMDM (A) and BMDM harboring ΔF508 mutation (ΔF508) (B) representing the colocalization of B. cepacia with LC3/Atg8I after 2 h infection. Nuclei are stained with DAPI, B. cepacia expresses red florescent protein and LC3/Atg8 is stained green with specific antibodies. White arrows indicate the presence of LC3/Atg8. (C) the percentage of colocalization of B. cepacia with LC3/Atg8 was scored. (D) transmission electron microscopy of murine BMDM expressing WT or mutated ΔF508 CFtr protein after 2 h infection with B. cepacia. Black arrow indicates a mutilamellar autophagosome-like vacuole. White arrow heads indicate vacuoles containing intact bacteria within single-membrane vacuoles. (E) the scores for the percentage of puncta in 100 WT and ΔF508 macrophages after 4 h infection with B. cepacia. Data are representative of 3 independent experiments and presented as the means ± SD. Asterisks in (C and E) indicate significant differences (*p < 0.05; **p < 0.01).