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. 2010 Nov 16;6(8):1090–1106. doi: 10.4161/auto.6.8.13426

Figure 9.

Figure 9

Mitochondria-associated proteins other than VDAC1 and VDAC3 are K63-polyubiquitinated in a Parkin-dependent manner; and VDAC1 and VDAC3 are dispensable for p62 recruitment to mitochondria, mitochondrial sequestration and Parkin-induced mitophagy. (A) VDAC1/3−/− mouse embryonic fibroblasts (MEFs) transiently expressing EYFP-Parkin (white) and treated with 20 µM CCCP for 3 hrs were immunostained for K63 polyubiquitinated proteins (green) and the mitochondrial protein, cytochrome c (red). (B) VDAC1/3+/+ and VDAC1/3−/− MEFs transiently expressing EYFP-Parkin (green) were treated with CCCP for 24 hrs and immunostained for the mitochondrial protein Tom20 (red). (C) Cells in (B) scored for mitochondrial phenotype. (D) Cells treated as in (B) were immunostained for p62 (green) and Tom20 (red). Scale bar in last image = 10 µm.