Fig. 6.

The MuSK/TrkA chimera, MMT, fails to induce complex AChR clusters on laminin substrate. (A) Schematic drawing of MuSK/WT and the MMT chimera. (B) MuSK^−/− myotubes expressing MuSK wild-type (WT) or MMT were cultured on laminin and stained with α-BGT. Forced-expression of MMT induces the formation of immature cluster morphologies only. Representative images of clusters are shown. Scale bar, 10 μm. (C) Quantification of the different cluster morphologies in MuSK/WT and MMT expressing myotubes. MMT was unable to induce complex AChR clusters. n > 15 (number of independent experiments: days 1, 2, 4, 5 = 3; day 3 = 2; day 6 = 4); error bars; S.E.M.