Fig. 6.

Elevated insulin secretion of hadh^−/− mice. Blood glucose (A) and plasma insulin (B) concentrations of hadh^+/+ and hadh^−/− mice in the fed, fasted, and postprandial state (16-h fasting and 2-h access to diet). Data represent means of 12–16 mice ± se; *, P < 0.05; #, P < 0.001. C, Elevated insulin secretion of isolated islets from hadh^−/− mice in response to glucose and fatty acids. Islets of hadh^+/+ and hadh^−/− mice that were kept on a standard diet were isolated as described in Materials and Methods. After an overnight incubation in RPMI medium islets were incubated with 2.8 or 16.7 mm glucose, with 2.8 mm glucose plus 35 mm KCl alone or in the presence of 0.3 mm palmitate for 1 h. Insulin secreted by the islets was detected in the medium by an ELISA as described in Materials and Methods; *, P < 0.05; #, P < 0.01.