Skip to main content
. 2012 May 24;3:84. doi: 10.3389/fneur.2012.00084

Figure 6.

Figure 6

Resveratrol does not suppress T cells in the periphery or CNS. Total cells were isolated from the spleens of individual EAE mice treated with PBS alone, 100 mg/kg RSV, 250 mg/kg RSV, or 250 SRT501 daily until day 30 post-immunization. (A) Freshly isolated cells stained with antibodies to CD4 and CD8 were analyzed by flow cytometry. Representative plot for one mouse in each treatment group shows identifiable populations of CD4+ and CD8+ T cells. (B) The average percentage of CD4+ and CD8+ T cells found in EAE spleens (N = 4 mice/treatment group), and the percentage of CD4+ cells that stained positive for IL-17 and IFN-gamma, is shown. No difference is observed between treatment groups. (C) Spinal cords from mice in each treatment group (N = 4) were pooled together to ensure sufficient numbers of inflammatory cells for flow cytometry. Less than 10% of spinal cord mononuclear cells from each treatment group were CD4+ T cells. Within the CD4+ T cell population, 5–15% were IL-17+ and 10–20% were IFN-gamma+, with too few cells present for statistical analysis.