Fig. 1.

Circadian Kiss1 expression in the AVPV peaks at the time of the LH surge in OVX+E₂ rats. A, Relative Kiss1 mRNA levels (gray line) and plasma LH (dashed line). Sample frequency is one per 6 h. Data represent mean ± sem. B, Representative autoradiographic films of coronal sections at the same AVPV level from animals killed at the indicated times and labeled with an antisense Kiss1 mRNA ³⁵S-tagged riboprobe. One-way ANOVA for Kiss1 levels, F = 0.023, P = 0.023, n = 4–6 per time point; *, Significantly different from circadian time (CT) 0, Tukey post hoc comparison, P = 0.05).