Fig. 3.
Changes in gene expression in BCa xenograft in the premenopausal model. Vehicle [control (Con)], calcitriol (Cal; 0.05 μg/mouse), and dietary vitamin D3 (Vit D) were administered to tumor-bearing intact female mice as described in Fig. 1. Total RNA was isolated from harvested tumors, and the mRNA levels of ERα, aromatase (A) COX-2, the PG receptors EP2 and FP (B), p21, IGFBP-3, Bax, and Bcl2 (C) were determined by quantitative RT-PCR as described in Materials and Methods. Relative mRNA expression for each gene in the tumors from the control mice was set at 1. Values represent mean ± se from six to 11 determinations. *, P < 0.05, **, P < 0.01, and ***, P < 0.001 compared with control.