Fig. 1.

Effect of APR stimuli on serum FGF21 levels and mRNA levels in mouse liver. Mice were injected with saline, LPS (5 mg/kg, ip), zymosan A (80 mg/kg, ip), or turpentine (100 μl/mouse, sc), and the animals were euthanized at 16 h. A, Serum FGF21 levels were measured by ELISA as described in Materials and Methods. B, Total RNA was isolated from liver tissue, cDNA was synthesized with reverse transcriptase, and quantitative real-time PCR was performed as described in Materials and Methods. FGF21 mRNA levels were measured. C, HMGCS2 mRNA levels were measured. Quantitative PCR data were normalized to 36B4 mRNA for all experiments. Data (mean ± se, n = 4) are expressed as a percentage of controls administered saline. *, P < 0.05; **, P < 0.01; ***, P < 0.001.