Table 2.
Quantitative analysis of immunoreactive cell bodies or fiber densities in rats injected with either Blank-SAP or NK3-SAP in the arcuate nucleus
| Immunohistochemical phenotype | Mid-level arcuate |
Posterior arcuate |
Total percent loss | ||
|---|---|---|---|---|---|
| Blank-SAP | NK3-SAP | Blank-SAP | NK3-SAP | ||
| NK3R | 7.9 ± 0.7 | 0.3 ± 0.2a | 13.4 ± 1.9 | 0.2 ± 0.1a | 98.1 ± 0.8 |
| NKB | 10.4 ± 1.1 | 0.6 ± 0.5a | 50.8 ± 3.6 | 3.1 ± 1.1a | 92.6 ± 2.2 |
| Kisspeptin | 6.4 ± 1.0 | 0.1 ± 0.1a | 31.4 ± 3.6 | 1.4 ± 0.6a | 94.8 ± 1.6 |
| Dynorphin | 11.0 ± 1.5 | 3.6 ± 0.5a | 24.0 ± 5.7 | 4.1 ± 1.0a | 76.5 ± 2.9 |
| POMC | 33.7 ± 3.8 | 37.2 ± 3.2 | 29.7 ± 2.1 | 36.0 ± 3.0 | 0 |
| NPY | 0.07 ± 0.01 | 0.06 ± 0.02 | 0.03 ± 0.003 | 0.03 ± 0.004 | 0 |
| GnRH | 0.04 ± 0.004 | 0.04 ± 0.008 | NA | NA | 0 |
Values represent mean ± sem NK3R, NKB, kisspeptin, dynorphin, and POMC values represent the average number of labeled neurons/unilateral arcuate section. NPY and GnRH values reflect average fiber density values (see Materials and Methods). NA, Not analyzed.
a
P < 0.01, Significantly different, Blank-SAP vs. NK3-SAP-treated animals, n = 5–11 rats/group.