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. Author manuscript; available in PMC: 2013 Mar 30.
Published in final edited form as: Biochem Biophys Res Commun. 2012 Feb 28;420(1):54–60. doi: 10.1016/j.bbrc.2012.02.114

Figure 2.

Figure 2

The effects of peroxynitrite treatment on CPS1 activity in mouse mitochondrial fractions. (A) The mitochondrial fractions were incubated with 1.0 mM peroxynitrite at 37°C for different time periods and subjected to the CPS1 activity assay. (B) The mitochondrial fractions were incubated with different concentrations of peroxynitrite at 37°C for 10 min and subjected to the CPS1 activity assay with or without 10 mM NAG. (C) The mitochondrial fractions were incubated with 1.0 mM peroxynitrite at 37°C for 10 min and subsequently incubated with or without 5.0 mM GSH at 37°C for 10 min, followed by the CPS1 activity assay with 10 mM NAG.