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. 2011 Oct 18;21(9):1455–1465. doi: 10.1089/scd.2011.0219

FIG. 5.

FIG. 5.

Individual mesenchymal cell clones have varying endothelial potential. (A) Single clones of fetal lung mesenchymal cells were isolated and expanded. Clone 10.2 represents a distinct subclone obtained from clone 10. Each clone was cultured in EGM-2 for 7 days. Flk-1 expression was measured by FACS. Clone 10 appeared to express higher levels of Flk-1 in response to EGM-2. Similar results were seen with the subclone 10.2. (B) Culturing clone 7 cells for 7 days in EGM-2 did not induce classic cobblestone morphology. (C, D) Flk-1 and α-SMA expression in clone 7 cells was lower than original cells, but culturing clone 7 cells in EGM-2 did increase Flk-1 expression and inhibit α-SMA expression (*P<0.05, n=4). (E) EGM-2 stimulated endothelial cell morphology. (F, G) Flk-1 and α-SMA expression in clone 10 cells was comparable to original cells, and EGM-2 increased Flk-1 expression to higher levels than clone 7 cells. Clone 10 cells had lower α-SMA expression over time in both DMEM and EGM-2 (*P<0.05, n=4).