FIG. 3.
Tracking of EdU-labeled ADSCs and identification of SDF-1 expression in the bladder. (A) EdU-positive ADSCs were located mainly in muscular and submucosal layers (white arrow). Please note that some EdU colocalized with SMA. Boxed areas in the ×100 graphs are shown in the corresponding ×1,000 graphs. (B) The number of EdU-positive ADSCs in the whole bladder was counted and calculated by computer software. They only accounted for a small fraction of original transplanted ADSCs. (C) Bladder tissue from DM+T group was stained with SDF-1 antibody. Expression of SDF-1 was clearly detected in the bladder tissue sections. ADSC, adipose tissue-derived stem cell; EdU, 5-ethynyl-2-deoxyuridine; SMA, smooth muscle actin; SDF-1, stromal cell-derived factor-1.