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. 2011 Oct 18;21(9):1391–1400. doi: 10.1089/scd.2011.0244

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Copyright 2012, Mary Ann Liebert, Inc.

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FIG. 4. — Quantification of apoptosis. (A) Only cells positive for both TUNEL and DAPI were considered positive for apoptosis (white arrow). Apoptotic cells were located predominantly in mucosal layer compared with muscular layer in all groups. (B) Quantification of the apoptotic nuclei per×200 magnification field was analyzed by Image Pro Plus software. Significantly less apoptosis was noted in groups that received an injection of ADSCs. (C) The percentage of apoptosis in cultured cells with different treatments was evaluated. (D) Gene Caspase-3 expression in cultured cells was analyzed with real-time PCR. NG: regular medium with normal glucose. HG: regular medium with high glucose. HG+PSMCs CM: rat penile smooth muscle cells conditioned medium with high glucose. HG+ADSCs CM: rat ADSCs conditioned medium with high glucose. DAPI, 4’,6-diamidino-2-phenylindole.