Table 1.
Polymerase Chain Reaction Primers
| Gene | Sequence | Product Size | |
|---|---|---|---|
| RT-PCR | |||
| Pou5F1 (Oct3/4) | F | CGA GGA GTC CCA GGA TAT GA | 446 |
| Pou5F1 (Oct3/4) | R | GCC GGT TAC AGA ACC ACA CT | |
| Sox2 | F | ACC AGC TCG CAG ACC TAC AT | 388 |
| Sox2 | R | CCC TCC CAA TTC CCT TGT AT | |
| Nanog | F | GCC CTG AGA AGA AAG AAG AG | 356 |
| Nanog | R | CTG ACT GCC CCA TAC TGG AA | |
| Pbgd | F | TAG CAT GCA AGA GAC CAT GC | 354 |
| Pbgd | R | GGC CGA AGT CTC AAC AAC TC | |
| hPAP | F | CTG ATG AAT GGG AGC AGT GGT GGA ATG | 360 |
| hPAP | R | GCA GAC ACT CTA TGC CTG TGT GGA G | |
| β-galactosidase | F | CGT CGT TTT ACA ACG TCG TGA C | 423 |
| β-galactosidase | R | CGC CGA GTT AAC GCC ATC | |
| Stella | F | TCC TAC AAC CAG AAA CAC TAG | 304 |
| Stella | R | GTG CAG AGA CAT CTG AAT GG | |
| Cdx2 | F | CAG GAG GAA AGC TGA GTT GG | 373 |
| R | TTC TCA CAG TGT CCG TGC TC | ||
| qRT-PCR | |||
| Pou5F1 (Oct3/4) | F | AGA ACC GTG TGA GGT GGA AC real-time | 132 |
| Pou5F1 (Oct3/4) | R | GCC GGT TAC AGA ACC ACA CT real-time | |
| EGFP | F | GAA GCA GCA CGA CTT CTT CAA real-time | 155 |
| EGFP | R | AAG TCG ATG CCC TTC AGC TC real-time | |
| Pbgd | F | GCA CGG CAG CTT AAT GAT GT real-time | 163 |
| Pbgd | R | CAA GGC CGA AGT CTC AAC AC real-time | |
| Cdx2 | F | CAG GAG GAA AGC TGA GTT GG | 373 |
| Cdx2 | R | TTC TCA CAG TGT CCG TGC TC | |
| D2Rat250-F1 | F | GTC CCT CTC CTG TCC CTC TC | 180/158/146 |
| D2Rat250-R1 | R | GAA GTC TGA ACG CTC ATG CA | 180/158/146 |