Table 2.
List of Rat Embryonic Stem Cell Lines That Were Derived and How They Have Been Characterized
| Cell line strain | Derivation conditions | Morphology | Sex | Tg? | OCT4 | NANOG | SOX2 | Karyotype | Tetraploid percentage | |
|---|---|---|---|---|---|---|---|---|---|---|
| Embryo-derived Stem Cell LINES | ||||||||||
| 1 | F344 β-galactosidase transgenic >10 other lines derived in bFGF/LIF/15% FBS | LIF/bFGF-S | Flat, individual | NO | Y | Y | No | Y | ||
| Embryonic Stem Cell LINES | ||||||||||
| 9 | F344 wild-type | 2i/LIF-SF | Round colonies | ND | Y | Y | Y | |||
| 11 | F344 wild-type | 2i/LIF-SF | Round colonies | M | Y | Y | Y | |||
| 12 | F344 wild-type | 2i/LIF-SF | Round colonies | ND | Y | Y | Y | |||
| 13 | F344 wild-type | 2i/LIF-SF | Round colonies | ND | Y | Y | Y | |||
| 38 | F344 wild-type | 2i/LIF-FBS | Round colonies | M | Y | Y | Y | |||
| 39 | F344 wild-type | 2i/LIF-FBS | Round colonies | F | Y | Y | Y | |||
| 40 | F344 wild-type | 2i/LIF-FBS | Round colonies | F | Y | Y | Y | |||
| 2 | F344 β-galactosidase transgenic | 2i/LIF-FBS | Round colonies | M | Y | Y | Y | Y | Euploid | 60% |
| 3 | F344 β-galactosidase transgenic | 2i/LIF-FBS | Round colonies | M | Y | Y | Y | Y | Aneuploid | 16% |
| 4 | F344 β-galactosidase transgenic | 2i/LIF-SF | Round colonies | M | Y | Y | Y | Y | ||
| 32 | F344 hPAP transgenic | 2i/LIF-SF | Round colonies | M | Y | Y | Y | Y | ||
| 33 | F344 hPAP transgenic | 2i/LIF-SF | Round colonies | M | Y | Y | Y | Y | ||
| 34 | F344 hPAP transgenic | 2i/LIF-SF | Round colonies | F | Y | Y | Y | Y | ||
| 48 | DA | 2i/LIF-FBS | Round colonies | M | Y | Y | Y | Euploid | 14% | |
| 49 | DA | 2i/LIF-FBS | Round colonies | F | ND | ND | ND | Euploid | 18% | |
| 50 | DA | 2i/LIF-FBS | Round colonies | F | Y | Y | Y | Euploid | 32% | |
| 51 | DA | 2i/LIF-FBS | Round colonies | F | Y | Y | Y | |||
| 52 | DA | 2i/LIF-FBS | Round colonies | M | Y | Y | Y | |||
| 53 | DA | 2i/LIF-FBS | Round colonies | M | Y | Y | Y | |||
| 54 | DA | 2i/LIF-FBS | Round colonies | M | Y | Y | Y | Euploid | 32% | |
| 55 | DA | 2i/LIF-FBS | Round colonies | F | Y | Y | Y | |||
| 56 | DA | 2i/LIF-FBS | Round colonies | F | Y | Y | Y | |||
| 57 | DA | 2i/LIF-FBS | Round colonies | M | Y | Y | Y | |||
| 58 | SD | 2i/LIF-SF | Round colonies | ND | ND | ND | ND | |||
| 60 | SD | 2i/LIF-SF | Round colonies | ND | ND | ND | ND | |||
| Sublines | Strain | Notes |
|---|---|---|
| 1.1 | F344 β-galactosidase transgenic | pCX |
| 2.1 | F344 β-galactosidase transgenic | pCX |
| 2.2 | F344 β-galactosidase transgenic | Neural |
| 3.1 | F344 β-galactosidase transgenic | pN1 |
| 3.2 | F344 β-galactosidase transgenic | pCX |
| 4.1 | F344 β-galactosidase transgenic | pN1 |
| 30 | F344 β-galactosidase transgenic | PE-like |
| 48.1 | DA | pCX |
| 53.1 | DA | pCX |
| 54.1 | DA | pCX |
LIF, leukemia inhibitory factor; F344, Fischer 344; Tg, transgenic; bFGF, basic fibroblast growth factor; DA, Dark Agouti; SD, Sprague-Dawley; 2i, 2 inhibitor.