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Western blot analysis of calpain 3. (A) Immunoblot of control and patient muscle samples [quadriceps (quad), gastrocnemius (gastroc) and diaphragm (dia)] labelled with NCL-CALP-2C4 and NCL-CALP-2A12 antibodies, directed against epitopes encoded by exons 1 and 8 of CAPN3, respectively. Myosin heavy chain (MHC) from post-blotted gel indicates the amount of protein loaded. (B) Densitometric analysis of calpain 3. y-axis = fold change in intensity.
