Table 1.
Primer data.
| Gene | Accession number | Fwd primer (5′–3′) | Rev. primer (5′–3′) | Amplicon size | PCR efficiency |
|---|---|---|---|---|---|
| arp (ref. gen) | AY255630 | GAAAATCATCCAATTGCTGGATG | CTTCCCACGCAAGGACAGA | 101 | 1,813 |
| ef1ab (ref. gene) | BG933853 | TGCCCCTCCAGGATGTCTAC | CACGGCCCACAGGTACTG | 59 | 2,013 |
| fatp | CA373015/AF023258 | TGGGAGCTTGTGGGTTCAA | ACTTTCATGAGGCGGATTGG | 64 | 2,243 |
| actb | BG933897 | CCAAAGCCAACAGGGAGAAG | AGGGACAACACTGCCTGGAT | 92 | 2,007 |
| gst | BQ036247 | ATTTTGGGACGGGCTGACA | CCTGGTGCTCTGCTCCAGTT | 81 | 2,07 |
| tuba | BT049768 | GAGCCAGCCAATCAGATGGT | TGCGCTTGGTCTTGATTGTG | 110 | 2,009 |
| gpx | BQ036408 | TTCTCCACCACACTGGGATCA | GGAAATGGCATCAAGTGGAATT | 101 | 2,011 |
| hsp-70 | BG933934 | CCCCTGTCCCTGGCTATTG | CACCAGGCTGGTTGTCTGAGT | 121 | 1,895 |
| cox2 | AJ238307 | CGTCCTGAGGCAGGAGCAT | TGAGGCGTGTGGTCTGGAA | 62 | 2,18 |
| cpt1 | AM230810 | CTTTGGGAAGGGCCTGATC | CATGGACGCCTCGTACGTTA | 121 | 1,917 |
| bcl-x | NM_001141086 | GCCTGGACGCAGTGAAAGAG | GGACGGCGTGATGTGTAGCT | 107 | 2,127 |
Full gene names: acidic ribosomal protein (arp), elongation factor 1 alpha b (ef1ab), fatty acid transport protein (fatp), β-actin (actb), glutathione-s-transferase (gst), tubulin α (tuba), glutathione peroxidase (gpx), heat shock protein 70 (hsp-70), cyclooxygenase 2 (cox2), carnitine palmitoyltransferase 1 (cpt1), and B-cell lymphoma x (bcl-x).