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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Curr Opin Struct Biol. 2012 Mar 21;22(2):217–224. doi: 10.1016/j.sbi.2012.03.001

Figure 3. Members of the Get3/ArsA fold family.

Figure 3

A. Cartoon representations of dimers of members of the ArsA fold viewed down the putative TA binding groove. The structures are the transition state S. cerevisiae Get3 (PDB ID: 2WOJ), the archaeal M. jannaschii (PDB ID: 3UG6, monomers color ramped) and M. thermoautotropicum Get3s (PDB ID: 3ZQ6, monomers light blue/pink), E. coli ArsA (PDB ID: 1F48, each pseudo-dimer color ramped with the helix filling the groove in brown), and the cyanobacterial homolog Nostoc sp. all4481 (PDB ID: 3IGF, monomers color ramped). The human α-crystallin structure (PDB ID: 2WJ7) has been aligned in gray to the equivalent cyanobacterial domain. Metals are shown as spheres and nucleotides as sticks. B. Surface representations of the structures in A rotated 90° with residues colored as in Fig. 2D. Some foreground residues have been removed for clarity and the truncation is colored grey. MjGet3 is shown for the archaeal homologs and the loop filling the groove has been removed in the ArsA surface calculation to show the comparable groove. C. An alignment of notable regions of the ArsA family proteins from ClustalW. Numbering and region names are based on the S. cerevisiae protein similar to Fig. 2C. A black bar identifies residues corresponding to α8. The sequences are ScGet3 (Saccharomyces cerevisiae UniProt ID: Q12154), HsTRC40 (Homo sapiens UniProt ID: O43681), MjGet3 (Methanocaldococcus jannaschii UniProt ID: Q58542), MtGet3 (Methanobacter thermoautotropicum UniProt ID: O27555) CtAFP (Chlorobium tepidum UniProt ID: Q46366), NosAFP (Nostoc sp PCC 7120 UniProt ID: Q8YNT0), SynAFP (Synechocystis sp. PCC 6803 UniProt ID: F7UTP7), AthAFP (Arabidopsis thaliana UniProt ID: Q6DYE4), PtriAFP (Populus trichocarpa or California poplar UniProt ID: B9HWM7) and EcArsA (Escherichia coli plasmid R773 N- and C-terminal domains UniProt ID: P08690). Numbers to the right are percent identity to ScGet3 excluding crystallin domains.