A. Representative immunoblots of phosphorylated (phospho) and total mTOR, Akt, AMPK and rpS6 proteins in the FHL from SED (left panels) or PRE animals (right panels). Tubulin is shown as a loading control and PRE expression is shown relative to control. B. Expression of total mTOR, Akt, AMPK, and rpS6 proteins in the FHL of PRE (white bars) and SED animals (black bars). C. Relative expression of phosphorylated to total mTOR, Akt, AMPK, and rpS6 proteins in the FHL of PRE (white bars) and SED animals (black bars). * indicates statistically significant difference between training groups (p<0.05).