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Decreased extracellular brain serotonin (5-HT) does not affect cortical barrel field (BF) formation. Despite the profound reduction of brain 5-HT in VMAT2-cKO mice (see text), the BF appears grossly normal at P5 (B), similar to wild type (A). (C), similarly in the Pet1-KO mice where brain 5-HT concentration is reduced by ~70%, BF pattern appears normal at P7. BF formation was visualized in flattened cortex preparations stained with a 5-HTT (SERT) antibody.
