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. 2012 Mar 25;109(7):1253–1262. doi: 10.1093/aob/mcs057

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© The Author 2012. Published by Oxford University Press on behalf of the Annals of Botany Company.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — Kinetics of tissue damage-dependent LIN and HNL activation in rubber tree leaves. Leaf tissue damage was achieved using a dissection pin. Samples from damaged leaf areas were taken 30 s, 5 min and 10 min after tissue damage. Intact tissue served as zero-point sample. Linamarase (LIN; or BGLU) as well as hydroxynitrile lyase (HNL) activity was determined in the soluble protein fraction of the samples. The activation of both enzymes is completed within less than 30 s after tissue damage occurs. Values given in the figure are means ± s.d. with n = 3 per treatment (intact, damaged 30 s, damaged 5 min, damaged 10 min) and sampling or repetition (grey scale code). Different rubber tree seedlings were used in sampling I and II or III and IV. Activation factor is the quotient of mean enzyme activity in samples taken from tissue 0·5, 5 and 10 min after damage and average enzyme activity in samples taken from intact tissue.