Skip to main content
. 2012 Mar 25;109(7):1253–1262. doi: 10.1093/aob/mcs057

Table 1.

Tissue damage-dependent activation of linamarase (LIN) and hydroxynitrile lyase (HNL) in rubber tree (H. brasiliensis) leaves

Activity [μkat (g f. wt)−1]
Sample Intact tissue s.d. Damaged tissue s.d. AF Mean AF
LIN B I 0·69 0·16 2·63 0·39 4 8
B II 0·23 0·03 3·11 0·70 14
B III 0·23 0·09 1·66 0·44 7
C I 0·69 0·13 4·65 0·57 7
C II 0·29 0·04 3·85 0·86 14
C IV 1·15 0·04 11·01 1·57 10
D I 0·86 0·16 6·02 1·44 7
D II 0·57 0·09 3·65 0·65 6
D V 1·33 0·08 8·82 0·21 7
HNL B VI 1·84 0·13 4·33 0·82 2·4 4
B VII 9·05 0·10 12·79 0·58 1·4
B VIII 2·54 0·22 9·70 0·32 3·8
C IX 6·03 0·78 9·11 0·64 1·5
C VI 1·18 0·56 3·35 0·22 2·8
C VIII 2·37 0·17 6·52 0·29 2·8
D IX 1·43 0·41 7·07 0·81 4·9
D IV 2·99 1·42 30·43 7·71 10·2
D VIII 1·33 0·08 8·82 0·21 6·6

Leaf tissue damage was achieved using a dissection pin. Linamarase (LIN; or BGLU) as well as hydroxynitrile lyase (HNL) activity was determined in the soluble protein fraction of samples from damaged and intact leaf areas. The activity of both enzymes increases up to ten-fold in response to tissue damage. Values given are means ± s.d. with n = 3 per treatment (intact and damaged), leaf stage (B, C, D) and seedling (I–IX). AF: activation factor (quotient of mean enzyme activity in samples taken from damaged tissue and mean enzyme activity in samples taken from intact tissue); mean AF: mean of all activation factors; f. wt: fresh weight.