Fig. 5.

Movement of symplasmic fluorescent dye, CF, in seed coats of non-bulging line ‘121’ (A, B) and a bulging line ‘282’ (C, D) at 6 (A, C) and 8 (B, D) dpa after 48 h dye feeding. Note, although CF appeared in seed coats of both genotypes at 6 dpa (A, C), particularly in the phloem unloading site at the chalazal end (arrowheads), a remarkable genotypic difference exists by 8 dpa (B vs. D): in line ‘282’ (D), CF was confined in seed coat phloem region (arrowheads in insert) and did not move to surrounding ground parenchyma cells. This contrasts with the extensive spread of CF throughout seed coats of line ‘121’ (B). pw, pod wall; sc, seed coat; em, embryo. Bar = 500 µm. Magnification of remaining images is the same as in (A).