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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: J Immunol. 2011 Sep 30;187(9):4913–4919. doi: 10.4049/jimmunol.1101000

Figure 2. Effect of OmCI on complement induced formation of C5a and TCC in vitro.

Figure 2

Left panel: Human whole blood preincubated with OmCI or equimolar amounts of albumin was incubated with 108 E. coli/ml for 30 minutes. Data are expressed as ng/ml and presented as mean ± SEM (n = 3).

Middle panels: Human and pig whole blood preincubated with OmCI or equimolar amounts of albumin was incubated with 108 E. coli/ml for 30 minutes at 37 °C. Data are expressed as AU/ml and presented as mean ± SEM (n = 3 for human and n = 4 for pig).

Right panels: Human and pig serum preincubated with 0.64 μM OmCI or albumin was activated with heat aggregated IgG (HAIGG) or zymosan, both at a final concentration of 1 mg/ml. In all experiments, zymosan induced TCC formation in pig whole blood was higher than the upper standard so these are set to 1.0. Data are expressed as TCC ratio, where 1.0 is defined as the amount of TCC formation induced by HAIGG and zymosan, respectively. The data are presented as mean ± SD (n = 5 for human and n = 3 for pig).