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. 2012 Apr;46(4):551–558. doi: 10.1165/rcmb.2011-0220OC

Figure 5.

Figure 5.

Effect of luminal hypertonicity and pH. (A) Luminal hypertonicity after the application of 100 mM glucose increased TEER by 27%, in line with earlier observations of the effects of luminal hypertonicity on other epithelia (20, 21). (B) A concomitant 26% decrease in albuterol permeability was observed in cells pretreated with luminal hypertonicity. (C) Effect of pH on overall albuterol transport in NHBE cells. The transport of albuterol was studied at pH 5.7, 7.4, and 8.2. A decrease in pH is known to change the proton acceptor sites in tight junctions and decrease the paracellular permeability of cations. As expected, a decrease in pH also decreases albuterol transport. (D) Effect of pH on albuterol uptake into cells. A lower pH also decreased the cellular uptake of albuterol. All data represent the mean ± SE for n = 4 experiments from two different lungs. *P < 0.05.