TABLE 1.
ARTERIAL BLOOD GASES, BRONCHOALVEOLAR LAVAGE DATA, AND HISTOLOGICAL ANALYSIS AT THE END OF 5 HOURS OF MECHANICAL VENTILATION USING TIDAL INFLATIONS OR DEEP INFLATIONS IN MICE THAT UNDERWENT PBS OR LPS ASPIRATION
| PBS + TI | PBS + DI | LPS + TI | LPS + DI | |
| Arterial blood gases | ||||
| PaO2/FiO2 | 289 (270–394) | 306 (265–339) | 268 (205–296) | 283 (274–339) |
| pH | 7.19 (7.15–7.30) | 7.34 (7.29–7.41)* | 7.17 (7.07–7.22)† | 7.24 (7.16–7.32) |
| PaCO2, mm Hg | 34 (23–40) | 22 (21–30) | 34 (31–48)† | 20 (13–24)‡ |
| HCO3−, mmol/L | 13.0 (11.3–14.0) | 13.2 (9.9–15.1) | 12.5 (11.0–14.5) | 9.1 (4.9–11.4) |
| Lactates, mmol/L | 2.5 (2.3–2.7) | 2.6 (2.5–3.0) | 2.8 (2.4–3.0) | 3.2 (2.7–4.1) |
| Bronchoalveolar lavage | ||||
| Cell count, cell/μl§ | 380 (105–845) | 1,080 (535–1225) | 2,255 (2005–2815)*† | 2,430 (2,047–2,800)*† |
| Protein concentration, μg/ml | 88 (22–145) | 63 (18–185) | 134 (120–233)* | 250 (150–470)*† |
| Lung histology | ||||
| Lung edema, % surface | 45 (43–47) | 43 (41–48) | 50 (46–52)† | 49 (46–50)*† |
| Vascular congestion, % surface | 75 (73–76) | 74 (73–75) | 79 (77–80) *† | 79 (77–80)*† |
Definition of abbreviations: DI, deep inflations; PaCO2, arterial carbon dioxide tension; PaO2/FiO2, ratio of arterial oxygen tension and fraction of inspired oxygen fraction; TI, tidal inflations.
Data are median (25th–75th percentile); n = 7–13 animals/group.
P value < 0.05 (Mann-Withney test after Kruskal Wallis test) as compared to PBS + TI.
P value < 0.05 (Mann-Withney test after Kruskal Wallis test) as compared to PBS + DI.
P value < 0.05 (Mann-Withney test after Kruskal Wallis test) as compared to LPS + TI.
Differential cell count was performed in a limited number of animals and showed a predominance of polymorphonuclear neutrophils (>80% of total cells) in all groups.