Figure 4.

Overexpression of FoxM1 is insufficient to induce HPASMC proliferation under normal conditions. HPASMCs were plated at equal density and transfected with a plasmid encoding T7-FoxM1 or an empty vector plasmid. Forty-eight hours after transfection, exogenous FoxM1 expression was determined by Western blot analysis with a T7 tag antibody (A), cell proliferation was measured by BrdU incorporation (B), and total cell numbers were counted using a hemocytometer. Data are expressed as mean ± SEM (n = 3). Ctrl, control.