Figure 6.

Suppression of FoxM1 does not alter mRNA expression of HIF-1α or HIF-2α, but reduces the induction of aurora A kinase and cyclin D1 during hypoxia. HPASMCs were plated at equal density and transfected with FoxM1-specific siRNA (siFoxM1). Twenty-four hours after exposure to normoxia or hypoxia, mRNA levels of FoxM1 (A), HIF-1α (B), HIF-2α (C), aurora A kinase (D), and cyclin D1 (E) were determined by quantitative RT-PCR. Results are shown as percent change over the group in which cells were transfected with negative control siRNA and exposed to normoxia (N/siNeg). Data are expressed as mean ± SEM (n = 3in A, D, and E, 4 in B, and 6 in C). *P < 0.05. **P < 0.01. n.s., no significance.